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Figure 1. Simply Blue Safe Stained SDS-PAGE analysis of mAb to cardiac troponin I, clone 10G1. 4-15% gradient gel is used for analysis. Lane 1. Monoclonal antibody to cardiac troponin I, 10G1 (-DTT) Lane 2. Size marker Lane 3. Monoclonal antibody to cardiac troponin I, 10G1(+DTT)
Figure 2. Analytical SEC of final product.
Figure 3. Octet RED96e analysis, antibody was loaded on sensor for capture of human cardiac troponin complex.
Figure 4. Octet RED96e analysis, biotinylated human cardiac troponin complex was loaded on sensor for capture of the antibody.Catalogue #
R1-130-100
Name
Human IgG1 monoclonal antibody to cardiac troponin I, clone 10G1
Description
The epitope for clone 10G1 corresponds to the following amino acid (aa) positions in the cTnI protein sequence: 1-16.
Target
Human cardiac troponin I
Target Description
Recombinant human cardiac troponin complex protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
Alternative Names
CMD1FF, CMD2A, CMH7, RCM1, TNNC1
Uniprot ID
P19429
Clonality
Human monoclonal
Clone
10G1
Class
hIgG1
Reactivity
Binds to human cardiac troponin I. Lack of cross-reactivity to skeletal troponin I was confirmed with ELISA using native human skeletal muscle troponin I.
Dissociation constant (KD)
2.50 x 10-09 M (troponin complex binding from solution)
8.0 x 10-12 M (troponin complex binding from streptavidin surface)
Application
ELISA, CLIA, WB
Protocol
Purification
Protein A affinity chromatography followed by gel filtration.
Concentration
1 mg/ml
Buffer
PBS, pH 7.4
QC
Simply Blue Safe stained SDS-PAGE, analytical HPLC-SEC, Octet binding.
Related Products
Cardiac troponin I antibody clone 10G1 has been identified as a recommended detection antibody with clone 10C12 (cat# R1-135-100) and clone 5F8 (cat# R1-126-100).
Shipping
Shipping on dry ice.
Storage
Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.
Background
Cardiac troponin I (cTnI), a 24 kDa protein, is a vital part of the troponin complex, working alongside troponin T and troponin C to control the contraction and relaxation of cardiac muscle fibres. In cases of cardiac injury or stress, such as myocardial infarction (MI), the integrity of cardiac muscle cells is compromised, leading to the release of cTnI into the bloodstream. Cardiac troponin I (cTnI) possesses a remarkable specificity for the heart muscle, rendering it an invaluable diagnostic tool in identifying myocardial infarction (MI). Consequently, cTnI has attained the status of the gold standard biomarker for diagnosing myocardial injury, with a particular emphasis on myocardial infarction. Given that the majority, if not all, of the cTnI molecules in the blood exist as binary (cTnI-C) or ternary complexes (cTnT-I-C), it's crucial to utilize cTnI-specific antibodies that can precisely recognize the troponin complex when designing an assay.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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