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Figure 1. Simply Blue Safe Stain SDS-PAGE analysis of monoclonal antibody to Influenza A, clone 3A9. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg monoclonal antibody to Influenza A, clone 3A9 (-DTT) Lane 2. Size marker Lane 3. 0.8 µg monoclonal antibody to Influenza A, clone 3A9 (+DTT)
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of H1N1 nucleocapsid protein.
Figure 5. Octet RED96e analysis, antibody was loaded on sensor for capture of H3N2 nucleocapsid protein.(for reference only; data shown are from a single production batch at release and may not be representative of other batches)
Catalogue #
R1-201-100
Name
Monoclonal antibody to Influenza A, clone 3A9
Target
Recombinant Influenza A Nucleocapsid protein
Target Description
Produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Source
Human
Clonality
Human monoclonal
Clone
3A9
Class
hIgG1
Reactivity
Recognizes recombinant NPs from subtypes H1N1 and H3N2, as well as inactivated viruses Influenza A/New Caledonia/20/99 (H1N1) and Influenza A/Panama/2007/99 (H3N2). Clone 3A9 shows some cross-reactivity at high concentrations to recombinant Influenza B NP from Influenza B/Florida/4/2006 and to inactivated viruses Influenza B/Malaysia/2506/04 and Influenza B/Florida/07/04. Additionally, no cross-reactivity to tested human parainfluenza virus (HPIV Type 3), respiratory syncytial viruses (RSV A, RSV B) and human metapneumoviruses (hMPV 9 Type A1, hMPV 3 Type B1, hMPV 18 Type B2) lysates was observed.
Application
ELISA
Protocol
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Related Products
Influenza A antibody clone 3A9 has been identified as a recommended recommended detection antibody with clone 5A4 (cat# R1-189-100).
Shipping
Shipped on dry ice.
Storage
Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.
Background
Influenza is an acute respiratory disease in mammals and domestic poultry that emerges from zoonotic reservoirs in aquatic birds and bats. Influenza viruses are enveloped negative-sense, single-stranded, fragmented RNA viruses that belong to the Orthomyxoviridae family. Influenza A and B cause annual epidemics and occasional pandemics of respiratory tract infections with a broad spectrum of disease severity ranging from asymptomatic to death. Influenza A viruses are subtyped on the bases of two surface glycoproteins: haemagglutinin (HA, H-number) and neuraminidase (NA, N-number). Influenza A viruses H1N1 and H3N2 currently cause most of the Influenza A epidemic diseases in humans according to WHO. Due to low-fidelity RNA polymerase proofreading capabilities, Influenza viruses have high mutation rate and are in a constant state of evolution. Influenza nucleoproteins (NP) that coat viral RNA are more conserved compared to surface proteins like HA and NA and are therefore the most common targets of commercially available influenza diagnostics.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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