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Figure 1. Simply Blue Safe Stain SDS-PAGE analysis of monoclonal antibody to Influenza B, clone 1A2. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg monoclonal antibody to Influenza B, clone 1A2 (-DTT). Lane 2. Size marker. Lane 3. 0.8 µg monoclonal antibody to Influenza B, clone 1A2 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
1
2
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture Flu b nucleocapsid protein (1) and H3N2 nucleocapsid protein (2).
(for reference only; data shown are from a single production batch at release and may not be representative of other batches)
Catalogue #
R1-202-100
Name
Monoclonal antibody to Influenza B, clone 1A2
Target
Recombinant Influenza B Nucleocapsid protein
Target Description
Recombinant Influenza B nucleoprotein produced in Baculovirus-Insect Cells (B/Florida/4/2006).
Source
Human
Clonality
Human monoclonal
Clone
1A2
Class
hIgG1
Reactivity
Recognizes recombinant Influenza B NP from Influenza B/Florida/4/2006 (Yamagata lineage) and inactivated viruses Influenza B/Malaysia/2506/04 (Victoria lineage) and Influenza B/Florida/07/04 (Yamagata lineage). Clone 1A2 shows no cross-reactivity to recombinant NPs from Influenza A subtypes H1N1 and H3N2, nor to inactivated viruses Influenza A/New Caledonia/20/99 (H1N1) and Influenza A/Panama/2007/99 (H3N2). No cross-reactivity to tested human parainfluenza virus (HPIV Type 3), respiratory syncytial viruses (RSV A, RSV B) and human metapneumoviruses (hMPV 9 Type A1, hMPV 3 Type B1, hMPV 18 Type B2) lysates was observed.
Application
ELISA
Protocol
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Related Products
Influenza B antibody clone 1A2 has been identified as a recommended capture antibody with clone 24C7 (cat# R1-208-100).
Shipping
Shipped with blue ice.
Storage
Store at +2…+8°C
Background
Influenza is an acute respiratory disease in mammals and domestic poultry that emerges from zoonotic reservoirs in aquatic birds and bats. Influenza viruses are enveloped negative-sense, single-stranded, fragmented RNA viruses that belong to the Orthomyxoviridae family. Influenza A and B cause annual epidemics and occasional pandemics of respiratory tract infections with a broad spectrum of disease severity ranging from asymptomatic to death. Influenza B viruses are classified into two circulating lineages: B/Victoria and B/Yamagata. Due to their low-fidelity RNA polymerase proofreading capabilities, Influenza viruses have a high mutation rate and are in a constant state of evolution. Influenza nucleoproteins (NP) that coat viral RNA are more conserved compared to surface proteins like HA and NA and are therefore the most common targets of commercially available influenza diagnostics.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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