Monoclonal antibody to Influenza B, clone 7D4

Catalogue #

R1-203-100

Name

Monoclonal antibody to Influenza B, clone 7D4

Target

Recombinant Influenza B Nucleocapsid protein

Target Description

Recombinant Influenza B nucleoprotein produced in Baculovirus-Insect Cells (B/Florida/4/2006).

Clonality

Human monoclonal

Clone

7D4

Class

hIgG1

Reactivity

Recognizes recombinant Influenza B NP from Influenza B/Florida/4/2006 (Yamagata lineage) and inactivated viruses Influenza B/Malaysia/2506/04 (Victoria lineage) and Influenza B/Florida/07/04 (Yamagata lineage). Clone 7D4 shows no cross-reactivity to recombinant NPs from Influenza A subtypes H1N1 and H3N2, nor to inactivated viruses Influenza A/New Caledonia/20/99 (H1N1) and Influenza A/Panama/2007/99 (H3N2). No cross-reactivity to tested human parainfluenza virus (HPIV Type 3), respiratory syncytial viruses (RSV A, RSV B) and human metapneumoviruses (hMPV 9 Type A1, hMPV 3 Type B1, hMPV 18 Type B2) lysates was observed.

Dissociation constant (K_D)

1.06E-12 M (soluble recombinant Influenza B nucleoprotein antigen).

Application

ELISA

Protocol

Purification

Protein A affinity chromatography followed by gel filtration.

Concentration

1 mg/ml

Buffer

PBS pH 7.4

QC

Coomassie stained SDS-PAGE, analytical HPLC-SEC, octet binding

Related Products

Influenza B antibody clone 7D4 has been identified as a recommended capture antibody with clone 24C7 (cat# R1-208-100).

Shipping

Shipped on dry ice.

Storage

Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.

Background

Influenza is an acute respiratory disease in mammals and domestic poultry that emerges from zoonotic reservoirs in aquatic birds and bats. Influenza viruses are enveloped negative-sense, single-stranded, fragmented RNA viruses that belong to the Orthomyxoviridae family. Influenza A and B cause annual epidemics and occasional pandemics of respiratory tract infections with a broad spectrum of disease severity ranging from asymptomatic to death. Influenza A viruses are subtyped on the bases of two surface glycoproteins: haemagglutinin (HA, H-number) and neuraminidase (NA, N-number). Influenza A viruses H1N1 and H3N2 currently cause most of the Influenza A epidemic diseases in humans according to WHO. Due to low-fidelity RNA polymerase proofreading capabilities, Influenza viruses have high mutation rate and are in a constant state of evolution. Influenza nucleoproteins (NP) that coat viral RNA are more conserved compared to surface proteins like HA and NA and are therefore the most common targets of commercially available influenza diagnostics.