Monoclonal antibody to C-reactive protein, clone 4D1, hIgG1

Catalogue #

R1-258-100

Name

Monoclonal antibody to C-reactive protein, clone 4D1, hIgG1

Target

Native human C-reactive protein

Target Description

Native human C-reactive protein purified from human pleural fluid.

Alternative Names

CRP, PTX1

Uniprot ID

P02741

Source

Human

Clonality

Human monoclonal

Clone

4D1

Class

hIgG1

Reactivity

Binds to human C-reactive protein. Lack of cross-reactivity to Human Serum amyloid P component confirmed with in-house CLIA using Human Serum amyloid P component.

Dissociation constant (K_D)

Summary of antibody screening to soluble native human CRP
KD, M: <1,0E-12

Application

ELISA, CLIA

Protocol

Purification

Protein A affinity chromatography followed by desalting

Purity

>90%

Concentration

1 mg/ml

Buffer

PBS, pH 7.4

QC

LabChip protein analysis, analytical HPLC-SEC, Octet binding.

Related Products

CRP antibody clone 4D1 has been identified as a recommended capture antibody with clone 2C7 (cat# R1-256-100), clone 4C11 (cat# R1-257-100), and as a recommended detection antibody with clone 4C11 (cat# R1-257-100), clone 5F5 (cat# R1-259-100). The antibody pair 4D1-4C11 was additionally validated in human serum.

Shipping

Shipping on dry ice.

Storage

Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.

Background

C-reactive protein (CRP) is a pentameric protein composed of five identical subunits, each consisting of 206 amino acid residues. It is primarily produced by the liver, in response to pro-inflammatory cytokines such as interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha). While CRP is not a specific biomarker for any particular condition, its levels can be elevated in a variety of conditions, including infections, autoimmune diseases and cancer. In addition, CRP has been associated with an increased risk of cardiovascular event, such as myocardial infarction (MI) and stroke. The American Heart Association (AHA) guidelines suggest measuring CRP levels as part of the assessment of cardiovascular disease (CVD) risk in some individuals, specifically those with an intermediate risk of CVD based on traditional risk factors, such as age, diabetes, blood pressure, cholesterol levels, and smoking status. Furthermore, studies show that the elevation of CRP varies between bacterial and viral infections, rapid increase and moderate delayed response, respectively. Therefore, CRP can be used as a marker for antibiotics treatment to lower the problem of overconsumption of antibiotics worldwide. In addition, CRP is utilized in veterinary diagnostics as a biomarker for inflammation. In animals, particularly in dogs and cats, CRP testing is increasingly used in clinical practice to aid in the diagnosis and monitoring of various medical conditions, such as viral and bacterial infection, sepsis, pyometra, surgical trauma as well as post-surgical infections.