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Figure 1. CE-SDS virtual gel output (LabChip GX) for monoclonal antibody to Cardiac troponin T, clone 3D3 under non-reduced (NR, left) and reduced (R, right) conditions.
Figure 2. Analytical SEC of final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet BLI analysis, antibody was loaded on sensor for capture of Cardiac troponin T protein in different concentrations.Catalogue #
R1-251-100
Name
Monoclonal antibody to Cardiac troponin T, clone 3D3, hIgG1
Target
Recombinant human cardiac troponin complex
Target Description
Recombinant human cardiac troponin complex protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
Alternative Names
CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT
Uniprot ID
P45379
Source
Human
Clonality
Human monoclonal
Clone
3D3
Class
hIgG1
Reactivity
Binds to human cardiac troponin T. Lack of cross-reactivity to skeletal troponin T was confirmed with ELISA using native human skeletal muscle troponin T.
Dissociation constant (KD)
Summary of antibody screening to soluble cardiac troponin I/T/C complex and cTnT using an Octet system.
KD, M
Recombinant cTnT-I-C complex 2,85E-09
Recombinant cTnT antigen 9,65E-12
Application
ELISA, CLIA
Protocol
Purification
Protein A affinity chromatography followed by desalting.
Purity
>90%
Concentration
1 mg/ml
Buffer
PBS, pH 7.4
QC
LabChip protein analysis, analytical HPLC-SEC, Octet binding.
Related Products
Cardiac troponin T antibody clone 3D3 has been identified as a recommended detection antibody with clone 1D3 (cat# R1-248-100). The antibody pair 1D3 – 3D3 was additionally validated using serum samples collected from myocardial infarction (MI) patients.
Shipping
Shipped on dry ice.
Storage
Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.
Background
Cardiac troponin T (cTnT) is a protein found in cardiac muscle cells, specifically in the contractile apparatus known as the thin filaments. It is one of three subunits of the troponin complex, along with cardiac troponin I (cTnI) and cardiac troponin C (cTnC). Troponin T binds to tropomyosin, a protein that covers the binding sites on actin, to facilitate muscle contraction. It is released into the bloodstream when cardiac muscle cells are damaged or die. cTnT is highly specific to cardiac muscle injury or death and is therefore a widely used biomarker for diagnosing acute myocardial infarction (AMI).
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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