Monoclonal antibody to CHI3L1, clone 4G3, hIgG1

Catalogue #

R1-233-100

Name

Monoclonal antibody to CHI3L1, clone 4G3, hIgG1

Target

Recombinant CHI3L1 protein

Target Description

Recombinant CHI3L1 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.

Alternative Names

YKL-40, chondrex, HC-gp39, HCGP39, cartilage glycoprotein 39, chitinase-3-like protein 1

Uniprot ID

P36222

Source

Human

Clonality

Human monoclonal

Clone

4G3

Class

hIgG1

Reactivity

Binds to CHI3L1 protein. Lack of cross-reactivity to CHI3L2, CHIA, and CHIT1 proteins was demonstrated.

Dissociation constant (K_D)

Summary of antibody screening to soluble CHI3L1 using an Octet system.
KD, M: 3,44E-10

Application

ELISA, CLIA

Protocol

Purification

Protein A affinity chromatography followed by desalting

Purity

>90%

Concentration

1 mg/ml

Buffer

PBS, pH 7.4

QC

LabChip protein analysis, analytical HPLC-SEC, Octet binding.

Related Products

CHI3L1 antibody clone 4G3 has been identified as a recommended capture and detection antibody with clone 13D10 (cat# R1-236-100).

Shipping

Shipped on dry ice.

Storage

Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.

Background

Chitinase-3-like protein 1 (CHI3L1) also known as YKL-40, Chondrex and HC-gp39 is a non-enzymatic protein that binds to chitin, heparin, and hyaluronic acid. CHI3L1 plays a crucial role in protecting against pathogens, antigen-induced and oxidant-induced injury responses, inflammation. It promotes tissue repair and remodelling by regulating a variety of essential biological processes. These include oxidant injury, apoptosis, pyroptosis, inflammasome activation, Th1/Th2 inflammatory balance, M2 macrophage differentiation, dendritic cell (DC) accumulation, TGF-β1 expression, ECM regulation, and parenchymal scarring. The potential of CHI3L1 as biomarker is being studied in connection to variety of diseases such as asthma, arthritis, sepsis, diabetes, liver fibrosis, coronary artery disease and cancers.