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Figure 1. CE-SDS virtual gel output (LabChip GX) for monoclonal antibody to D-dimer, clone 9D2 under non-reduced (NR, left) and reduced (R, right) conditions.
Figure 2. Analytical SEC of final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet BLI analysis, antibody was loaded on sensor for capture of D-dimer protein in different concentrations.Catalogue #
R1-254-100
Name
Monoclonal antibody to D-dimer, clone 8G5, hIgG1
Target
Native human D-dimer protein
Target Description
Native human D-dimer protein purified from human plasma.
Alternative Names
Dimerized plasmin fragment D, D dimer
Source
Human
Clonality
Human monoclonal
Clone
8G5
Class
hIgG1
Reactivity
Binds to D-dimer, high molecular weight FDP (FDP-X)., and soluble fibrinogen. Lack of cross-reactivity low molecular weight FDP-E confirmed with Octet analysis.
Dissociation constant (KD)
Summary of antibody screening to soluble D-dimer using an Octet system.
KD, M: <1,0E-12
Application
ELISA, CLIA
Protocol
Purification
Protein A affinity chromatography followed by desalting.
Purity
>90%
Concentration
1 mg/ml
Buffer
PBS, pH 7.4
QC
LabChip protein analysis, analytical HPLC-SEC, Octet binding.
Related Products
D-dimer antibody clone 8G5 has been identified as a recommended detection antibody with clone 2H6 (cat# R1-252-100), clone 8G2 (R1-253-100), and clone 9D2 (cat# R1-255-100).
Shipping
Shipping on dry ice.
Storage
Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.
Background
Upon vascular damage, due to injury or underlying disease, blood coagulation cascade initiates the formation of fibrin clots from fibrinogen, which in turn is degraded in fibrinolysis. The fibrinogen molecule consists of E-domains and cross-linked D-domains. During fibrinolysis, fibrinogen is enzymatically digested into variable length fibrin degradation products (FDPs) where D-dimer is one of the smallest proteins and in blood samples still non-covalently bound to the E-domain. Therefore, the detection of elevated D-dimer levels indicates increased thrombotic activity in the body that can be caused by most cardiovascular diseases, like disseminated intravascular coagulation, myocardial infarction (MI), and stroke. Despite the non-specificity of D-dimer as a biomarker, high D-dimer level is used in diagnostics to exclude the diagnosis of deep vein thrombosis (DVT).
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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