Monoclonal antibody to D-dimer, clone 9D2, hIgG1

Catalogue #

R1-255-100

Name

Monoclonal antibody to D-dimer, clone 9D2, hIgG1

Target

Native human D-dimer protein

Target Description

Native human D-dimer protein purified from human plasma.

Alternative Names

Dimerized plasmin fragment D, D dimer

Clonality

Human monoclonal

Clone

9D2

Class

hIgG1

Reactivity

Binds to D-dimer and high molecular weight FDP (FDP-X). Lack of cross-reactivity to soluble fibrinogen and low molecular weight FDP-E confirmed with Octet analysis.

Dissociation constant (K_D)

Summary of antibody screening to soluble D-dimer using an Octet system.
KD, M: 1,90E-10

Application

ELISA, CLIA

Protocol

Purification

Protein A affinity chromatography followed by desalting.

Purity

>90%

Concentration

1 mg/ml

Buffer

PBS, pH 7.4

QC

LabChip protein analysis, analytical HPLC-SEC, Octet binding.

Related Products

D-dimer antibody clone 9D2 has been identified as a recommended capture antibody with clone 8G5 (cat# R1-254-100) and clone 2H6 (R1-252-100).

Shipping

Shipping on dry ice.

Storage

Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.

Background

Upon vascular damage, due to injury or underlying disease, blood coagulation cascade initiates the formation of fibrin clots from fibrinogen, which in turn is degraded in fibrinolysis. The fibrinogen molecule consists of E-domains and cross-linked D-domains. During fibrinolysis, fibrinogen is enzymatically digested into variable length fibrin degradation products (FDPs) where D-dimer is one of the smallest proteins and in blood samples still non-covalently bound to the E-domain. Therefore, the detection of elevated D-dimer levels indicates increased thrombotic activity in the body that can be caused by most cardiovascular diseases, like disseminated intravascular coagulation, myocardial infarction (MI), and stroke. Despite the non-specificity of D-dimer as a biomarker, high D-dimer level is used in diagnostics to exclude the diagnosis of deep vein thrombosis (DVT).