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Figure 1. CE-SDS virtual gel output (LabChip GX) for monoclonal antibody to FGF-21, clone 5D5 under non-reduced (NR, left) and reduced (R, right) conditions.
Figure 2. Analytical SEC of final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet BLI analysis, antibody was loaded on sensor for capture of FGF-21 protein in different concentrations.Catalogue #
R1-230-100
Name
Monoclonal antibody to FGF-21, clone 5D5, hIgG1
Target
Recombinant FGF-21 protein
Target Description
Recombinant FGF-21 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
Alternative Names
FGF21, fibroblast growth factor 21
Uniprot ID
Q9NSA1
Source
Human
Clonality
Human monoclonal
Clone
5D5
Class
hIgG1
Reactivity
Binds to FGF-21 protein. Lack of cross-reactivity to FGF19 and FGF23 was demonstrated with ELISA.
Dissociation constant (KD)
Summary of antibody screening to soluble FGF-21 using an Octet system.
KD, M: 2,71E-09
Application
ELISA, CLIA
Protocol
Purification
Protein A affinity chromatography followed by desalting
Purity
>90%
Concentration
1 mg/ml
Buffer
PBS, pH 7.4
QC
LabChip protein analysis, analytical HPLC-SEC, Octet binding
Related Products
FGF-21 antibody clone 5D5 has been identified as a recommended detection antibody with clone 4F3 (cat# R1-228-100) and as a recommended capture antibody with clone 9F5 (cat#R1-231-100).
Shipping
Shipped on dry ice.
Storage
Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.
Background
Fibroblast growth factor 21 (FGF21) is a protein hormone synthesized mainly in the liver. It regulates glycose and lipid metabolism and contributes to the energy balance. FGF21 has been suggested as a promising biomarker for several diseases like non-alcoholic fatty liver disease (NAFLD), heart failure, obesity, type II diabetes, diabetic nephropathy, renal disease, mitochondrial disease, and others. FGF21-based therapies are under development for non-alcoholic steatohepatitis (NASH) and related metabolic diseases.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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