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Figure 1. CE-SDS virtual gel output (LabChip GX) for monoclonal antibody to FGF-21, clone 9F5 under non-reduced (NR, left) and reduced (R, right) conditions.
Figure 2. Analytical SEC of final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet BLI analysis, antibody was loaded on sensor for capture of FGF-21 protein in different concentrations.Catalogue #
R1-231-100
Name
Monoclonal antibody to FGF-21, clone 9F5, hIgG1
Target
Recombinant FGF-21 protein
Target Description
Recombinant FGF-21 protein produced by CHO-based cell line (expressed by QMCF Technology).
Alternative Names
FGF21, fibroblast growth factor 21
Uniprot ID
Q9NSA1
Source
Human
Clonality
Human monoclonal
Clone
9F5
Class
hIgG1
Reactivity
Binds to FGF-21 protein. Lack of cross-reactivity to FGF19 and FGF23 was demonstrated with ELISA.
Dissociation constant (KD)
Summary of antibody screening to soluble FGF-21 using an Octet system.
KD, M: 7,97E-09
Application
ELISA, CLIA
Protocol
Purification
Protein A affinity chromatography followed by desalting
Purity
>90%
Concentration
1 mg/ml
Buffer
PBS, pH 7.4
QC
LabChip protein analysis, analytical HPLC-SEC, Octet binding
Related Products
FGF-21 antibody clone 9F5 has been identified as a recommended detection antibody with clone 1E5 (cat# R1-227-100) and clone 5D5 (cat# R1-230-100).
Shipping
Shipped on dry ice.
Storage
Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.
Background
Fibroblast growth factor 21 (FGF21) is a protein hormone synthesized mainly in the liver. It regulates glycose and lipid metabolism and contributes to the energy balance. FGF21 has been suggested as a promising biomarker for several diseases like non-alcoholic fatty liver disease (NAFLD), heart failure, obesity, type II diabetes, diabetic nephropathy, renal disease, mitochondrial disease, and others. FGF21-based therapies are under development for non-alcoholic steatohepatitis (NASH) and related metabolic diseases.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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