Monoclonal antibody to Influenza A, clone 5A4

Catalogue #

R1-189-100

Name

Monoclonal antibody to Influenza A, clone 5A4

Target

Recombinant Influenza A Nucleocapsid protein

Target Description

Produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line

Uniprot ID

P03466

Source

Human

Clonality

Human monoclonal

Clone

5A4

Class

hIgG1

Reactivity

Recognizes recombinant NPs from subtypes H1N1 and H3N2, as well as inactivated viruses Influenza A/New Caledonia/20/99 (H1N1) and Influenza A/Panama/2007/99 (H3N2). Clone 5A4 demonstrated no cross-reactivity to recombinant Influenza B NP from Influenza B/Florida/4/2006 and to inactivated viruses Influenza B/Malaysia/2506/04 and Influenza B/Florida/07/04. Additionally, no cross-reactivity to tested human parainfluenza virus (HPIV Type 3), respiratory syncytial viruses (RSV A, RSV B) and human metapneumoviruses (hMPV 9 Type A1, hMPV 3 Type B1, hMPV 18 Type B2) lysates was observed.

Dissociation constant (K_D)

5,41E-10 M (soluble recombinant Influenza A nucleoprotein antigen from H1N1 subtype).
2,07E-12 M (soluble recombinant Influenza A nucleoprotein antigen from H3N2 subtype).

Application

ELISA

Protocol

Purification

Protein A affinity chromatography followed by gel filtration

Concentration

1 mg/ml

Buffer

PBS pH 7.4

QC

LabChip protein analysis, analytical HPLC-SEC

Related Products

Influenza A antibody clone 5A4 has been identified as a recommended capture antibody with clone 3A9 (cat# R1-201-100), and as a recommended detection antibody with clone 8F8 (cat# R1-190-100).

Shipping

Shipped with blue ice.

Storage

Store at +2…+8°C.

Background

Influenza is an acute respiratory disease in mammals and domestic poultry that emerges from zoonotic reservoirs in aquatic birds and bats. Influenza viruses are enveloped negative-sense, single-stranded, fragmented RNA viruses that belong to the Orthomyxoviridae family. Influenza A and B cause annual epidemics and occasional pandemics of respiratory tract infections with a broad spectrum of disease severity ranging from asymptomatic to death. Influenza A viruses are subtyped on the bases of two surface glycoproteins: haemagglutinin (HA, H-number) and neuraminidase (NA, N-number). Influenza A viruses H1N1 and H3N2 currently cause most of the Influenza A epidemic diseases in humans according to WHO. Due to low-fidelity RNA polymerase proofreading capabilities, Influenza viruses have high mutation rate and are in a constant state of evolution. Influenza nucleoproteins (NP) that coat viral RNA are more conserved compared to surface proteins like HA and NA and are therefore the most common targets of commercially available influenza diagnostics.