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Monoclonal antibody to Leptin, clone 2B3, hIgG1

Figure 1. CE-SDS virtual gel output (LabChip GXII) for monoclonal antibody to Leptin, clone 2B3 under non-reduced (NR, left) and reduced (R, right) conditions. Figure 2. Analytical HPLC-SEC of final product. Figure 3. Analytical HPLC-SEC after 3 freeze-thaw cycles. Figure 4. Octet BLI analysis, antibody was loaded on sensor for capture of Leptin protein in different concentrations.

(for reference only; data shown are from a single production batch at release and may not be representative of other batches)

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Catalogue #

R1-280-100

Name

Monoclonal antibody to Leptin, clone 2B3, hIgG1

Target

Recombinant leptin protein

Target Description

Recombinant Leptin protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.

Alternative Names

LEPD, OB, OBS

Uniprot ID

P41159

Clonality

Human monoclonal

Clone

2B3

Class

hIgG1

Reactivity

Binds to Leptin protein

Application

ELISA, CLIA

Protocol

Monoclonal antibody working amount has to be established practically for each particular antigen and assay format.

Concentration

1 mg/ml

Buffer

PBS, pH 7.4, Proclin 0.05%

Related Products

Leptin antibody clone 2B3 has been identified as a recommended capture antibody with clone 6F7 (cat# R1-281-100). The antibody pair 2B3-6F7 was additionally validated in human serum.

Shipping

Shipping on blue ice.

Storage

Store at +2 °C to +8 °C.

Background

Leptin is a 16 kDa protein hormone mainly secreted from the adipose tissue. Its main function is to bind with receptors in the hypothalamus which results in suppression of hunger, stimulation of satiety and increase in energy expenditure. In addition to its role in energy homeostasis, leptin is involved in various physiological processes, including reproduction, immunity, and bone metabolism. Leptin might serve as a novel biomarker since it is associated with diseases such as obesity, cardiovascular diseases, non-alcoholic fatty liver disease (NAFLD), and neurodegenerative diseases.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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