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Figure 1. CE-SDS virtual gel output (LabChip GX) for monoclonal antibody to NGAL, clone 6D1 under non-reduced (NR, left) and reduced (R, right) conditions.
HPLC analytical SEC
Figure 2. HPLC analytical SEC
HPLC analytical SEC after 3 freeze-thaw cycles
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Octet binding analysis
Figure 4. Octet BLI analysis, antibody was loaded on sensor for capture of NGAL protein in different concentrations.(for reference only; data shown are from a single production batch at release and may not be representative of other batches)
Catalogue #
R1-324-100
Name
Monoclonal antibody to NGAL, clone 6D1, hIgG1
Target
Recombinant NGAL protein
Target Description
Recombinant NGAL protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
Alternative Names
Neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, lipocalin-2, oncogene 24p3, siderocalin, p25, LCN2, HNL.
Uniprot ID
P80188
Clonality
Human monoclonal
Clone
6D1
Class
hIgG1
Reactivity
Binds to NGAL protein
Application
ELISA, CLIA
Protocol
Concentration
1 mg/ml
Buffer
PBS, pH 7.4
Related Products
NGAL antibody clone 6D1 has been identified as a recommended capture antibody with clone 5B12 (cat# R1-309-100). The antibody pair 6D1-5B12 was additionally validated using human serum samples.
Shipping
Shipped on dry ice.
Storage
Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.
Background
Neutrophil Gelatinase-Associated Lipocalin (NGAL), also known as lipocalin-2 (LCN2), is a small glycoprotein involved in various physiological and pathological processes. It plays a significant role in the body’s immune response and has emerged as an important biomarker for several diseases, particularly kidney-related conditions. NGAL concentrations in human blood provide crucial information for the early detection, diagnosis, and monitoring of various acute and chronic medical conditions such as acute kidney injury (AKI) and chronic kidney disease (CKD).
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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