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Figure 1. CE-SDS virtual gel output (LabChip GX) for monoclonal antibody to PAI-1, clone 10H12 under non-reduced (NR, left) and reduced (R, right) conditions.
Figure 2. Analytical SEC of final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet binding analysis, antibody was loaded on sensor for capture of PAI-1 protein in different concentrations.Catalogue #
R1-221-100
Name
Monoclonal antibody to PAI-1, clone 10H12, hIgG1
Target
Recombinant PAI-1 protein
Target Description
Recombinant PAI-1 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
Alternative Names
Plasminogen activator inhibitor-1, Serpin E1, PLANH1, endothelial plasminogen activator inhibitor, SERPINE1.
Uniprot ID
P05121
Source
Human
Clonality
Human monoclonal
Clone
10H12
Class
hIgG1
Reactivity
Binds to PAI-1 protein.
Dissociation constant (KD)
Summary of antibody screening to soluble PAI-1 using an Octet system.
KD, M: 1.75E-09
Application
ELISA, CLIA
Protocol
Kd:
2.809 x 10-09 M
Purification
Protein A affinity chromatography followed by desalting
Purity
>95%
Concentration
1 mg/ml
Buffer
PBS, pH 7.4
QC
LabChip protein analysis, analytical HPLC-SEC, Octet binding.
Related Products
PAI-1 antibody clone 10H12 has been identified as a recommended detection antibody with clone 9G6 (cat# R1-225-100) and clone 11D6 (cat# R1-222-100), and as a capture antibody with clone 11E1 (cat# R1-226-100).
Shipping
Shipped on dry ice.
Storage
Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.
Background
Plasminogen activator inhibitor-1 (PAI-1) also known as Serpin E1, is a serine protease inhibitor and important regulator of plasminogen/plasmin system. PAI-1 has been linked to many (patho)physiological conditions such as cardiovascular disease (CVD), metabolic disturbances, aging, cancer, tissue fibrosis, inflammation, and neurodegenerative diseases. PAI-1 has great potential as a biomarker for the different disease classes as mentioned above, and a drug target in cardiovascular diseases in particular.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2026 Icosagen
