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Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Nucleocapsid protein, clone 45B3. 10% gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Nucleocapsid protein, clone 45B3 (+DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Nucleocapsid protein, clone 45B3 (-DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Nucleocapsid protein in different concentrations (200, 100, 50 and 25 nM).
Catalogue #
R1-168-100
Name
Human IgG1-lambda antibody to SARS CoV-2 NP (clone 45B3)
Target
Nucleocapsid protein SARS-CoV-2
Target Description
Recombinant SARS-CoV-2 Nucleocapsid protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Uniprot ID
P0DTC9
Clonality
Human monoclonal
Clone
45B3
Class
hIgG1
Reactivity
SARS-CoV-2 Nucleocapsid protein
Dissociation constant (KD)
KD, M: 4.14E-10
Application
ELISA
Protocol
Purification
Protein A affinity chromatography followed by gel filtration
Concentration
1 mg/ml
Buffer
PBS pH 7.4
QC
SDS-PAGE, analytical HPLC-SEC, Octet binding, ELISA
Shipping
Shipped on dry ice.
Storage
Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com