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Human IgG1-lambda antibody to SARS-CoV-2 S1 RBD (clone 49F9)

 

Figure 1. Simply Blue Safe stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 49F9. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 49F9 (-DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 49F9 (+DTT).

       

Figure 2. HPLC analytical SEC for final product.

       

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

       

Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein in different concentrations (200, 100, 50, 25 and 12.5 nM).

       

Figure 5. Octet RED96e analysis, antibody was loaded on sensor for capture of Trimeric Spike protein in different concentrations (50, 25, 12.5, 6.25 and 3.13 nM).

(for reference only; data shown are from a single production batch at release and may not be representative of other batches)

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Catalogue #

R1-169-100

Name

Human IgG1-lambda antibody to SARS-CoV-2 S1 RBD (clone 49F9)

Target

SARS-CoV-2 virus

Target Description

SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology).

Alternative Names

Spike glycoprotein, S glycoprotein

Uniprot ID

P0DTC2

Clonality

Human monoclonal

Clone

49F9

Class

hIgG1

Reactivity

SARS-CoV-2 Spike S1 RBD, also recognized SARS CoV-2 Trimeric Spike in Octet analysis.

Application

ELISA

Protocol

Monoclonal antibody working amount has to be established practically for each particular antigen and assay format.

Concentration

1 mg/ml

Buffer

PBS pH 7.4

Shipping

Shipped on dry ice.

Storage

Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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