Mouse monoclonal antibody to bovine DNase I (clone 6D5)

Catalogue #

405-100

Name

Mouse monoclonal antibody to bovine DNase I (clone 6D5)

Target

Bovine DNase I

Target Description

Recombinant full length bovine DNaseI produced using CHO-based Icosagen Cell Factory Ltd. proprietary suspension cell line. Antigen is purified from cell culture supernatant.

Alternative Names

DNL1, Dnase1, Deoxyribonuclease-1, Deoxyribonuclease I

Uniprot ID

P00639

Clonality

Mouse monoclonal

Clone

6D5

Class

mIgG1

Reactivity

Bovine, cross reactivity to Human

Application

ELISA, WB, IF

Protocol

ELISA:

0,01-0,02 µg/ml

WB:

0,1-0,2 µg/ml

IF:

0,33-20 µg/ml

Purification

Protein G affinity chromatography followed by desalting.

Concentration

1 mg/ml

Buffer

PBS pH 7.4, with 0.1% sodium azide

QC

SDS-page, analytical HPLC-SEC, ELISA

Shipping

Shipping in ambient conditions.

Storage

Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.

Background

Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments.