Mouse monoclonal antibody to BPV type 1 E2 protein (clone 1H10)

Catalogue #

A1-155-100

Name

Mouse monoclonal antibody to BPV type 1 E2 protein (clone 1H10)

Target

BPV type 1 E2

Target Description

Recombinant full length BPV type I E2 protein produced in E. coli

Alternative Names

Regulatory protein E2, bovine papillomavirus type 1 E2

Uniprot ID

P03122

Clonality

Mouse monoclonal

Clone

1H10

Class

IgG

Reactivity

Bovine, BPV type 1 E2 protein, mapped to amino acids 208-218 (PPDGVWASEG)

Application

ELISA, WB, IF

Protocol

ELISA:

0,2-1 µg/ml

WB:

0,2-1 µg/ml

IF:

1 µg/ml

Purification

Protein A affinity chromatography

Buffer

PBS pH 7.4, 50% glycerol

Shipping

Shipping in ambient conditions.

Storage

Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.

Background

E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV.

References

Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999.
Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77.

Kurg R, Tekkel H, Abroi A, and Ustav M. 2006.
Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25