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Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (5E11).
A:Testing of purified recombinant BPV E2. Different amount of purified protein was loaded into the gel. Lane 1. 30 ng; Lane 2. 15 ng; Lane 3. 10 ng; Lane 4. 5 ng; Lane 5. 1 ng.
B: Testing of BPV E2 expressed in CHO cell line. Lane 1. Cell lysate of CHO cells. Lane 2. Cell lysate of CHO cells stably expressing BPV E2
(for reference only; data shown are from a single production batch at release and may not be representative of other batches)
Catalogue #
A1-100-100
Name
Mouse mAb to BPV type 1 E2-protein (clone 5E11)
Target
BPV type 1 E2
Target Description
Recombinant full length BPV type I E2 protein produced in E. coli
Alternative Names
Regulatory protein E2, bovine papillomavirus type 1 E2
Uniprot ID
P03122
Clonality
Mouse monoclonal
Clone
5E11
Class
IgG
Reactivity
Bovine, BPV type 1 E2 protein amino acids 199-208 (SSTSSDFRDR)
Application
ELISA, WB, IF
Protocol
Buffer
PBS pH 7.4, 50% glycerol
Shipping
Shipping in ambient conditions.
Storage
Store at -15…-25 °C. Avoid multiple freeze-thaw cycles.
Background
E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV.
References
Koljak R, Järving I, Kurg R, Boeglin WE, Varvas K, Valmsen K, Ustav M, Brash AB, and Samel N. 2001.
The basis of prostaglandin synthesis in coral, J. Biol. Chem., 276: 7033-7040.
Nigul L, Olspert A, Meier M, Paves M, Talpsep T, and Truve E. 2004.
New Plant vectors for Protein Tagging with E2 Epitope, Plant Molecular Biology Reporter, 4: 399 – 407.
Mannan
AU, Boehm J, Sauter SM, Rauber A, Byrne PC, Neesen J, and Engel W. 2006. Spastin, the most commonly mutated protein in hereditary
spastic paraplegia interacts with reticulon 1 an endoplasmic reticulum protein.
Neurogenetics 7: 93-103.
This product is for research use only
TECHNICAL ASSISTANCE
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