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Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (1E2). Testing BPV E2 protein-expressing Cos7 cell lysate. Different antibody dilutions were used for testing. Lane 1:500; Lane 2. 1:1000; Lane 3. 1:4000; Lane 4. 1:6000
A.
B.
Figure 2. Immunofluorescence testing of anti-BPV E2 monoclonal antibody (1E2). Assay was carried out with U2OS cells expressing BPV E2 protein. Antibody concentration of 0.33 µg/ml was used. Nuclei were stained by DAPI. Fluorescence was measured at (A) 460 nm and 488 nm wavelengths or (B) 488 nm only.
Catalogue #
A1-150-100
Name
Mouse mAb to BPV type 1 E2 protein (clone 1E2)
Target
BPV type 1 E2
Target Description
Recombinant full length BPV type I E2 protein produced in E. coli
Alternative Names
Regulatory protein E2, bovine papillomavirus type 1 E2
Uniprot ID
P03122
Clonality
Mouse monoclonal
Clone
1E2
Class
IgG
Reactivity
Bovine, BPV type 1 E2 protein, mapped to amino acids 182-190 (TTGHYSVRD)
Application
ELISA, WB, IF
Protocol
ELISA:
0.2 - 1 µg/ml
WB:
0.5 - 2 µg/ml
IF:
0.33 - 1 µg/ml
Purification
Protein A affinity chromatography followed by gel filtration and desalting.
Buffer
PBS pH 7.4, 50% glycerol
QC
SDS-page, analytical HPLC-SEC
Related Products
Monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: shop.icosagen.com/products/?antibodies
Shipping
Shipping in ambient conditions.
Storage
Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.
Background
E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV.
References
Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999.
Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77.
Kurg R, Tekkel H, Abroi A, and Ustav M. 2006.
Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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