Mouse monoclonal antibody to HIV-1 Rev (clone 10)

Catalogue #

A2-832-100

Name

Mouse monoclonal antibody to HIV-1 Rev (clone 10)

Target

HIV-1 Rev

Target Description

Recombinant Rev protein of HIV-1 BRV produced in E. coli)

Clonality

Mouse monoclonal

Clone

10

Class

mIgG1

Reactivity

Reacts with HIV-1 Rev of subtype B. Mapped to amino acids 34-40 (TRQARRN – subtype B a.a. consensus)

Application

ELISA, WB

Protocol

ELISA:

0,25-0,5 µg/ml

WB:

2 µg/ml

Purification

Protein G affinity chromatography

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

Shipping in ambient conditions.

Storage

Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.

References

Ranki A., A. Lagerstedt, V. Ovod, E. Aavik, and K. Krohn 1994.
Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef and Tat in established lymphoid cell lines. Arch. Virol., 139:365-378.

Ranki A., M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn 1995.
Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Tähtinen, M., Ranki, A., Valle,S.L., Ovod, V., K. Krohn 1997.
B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother 51:480-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren 2001.
Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1):46-61