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Mouse monoclonal antibody to HPV11 E2 (clone 4H1)

               

Figure 1.  Western-Blot analysis of HPV-11 E2 protein. WB was was carried out with 4H1 Mab using the lysates of U20S and Hela cells. Lines 1 and 4 are according cells transfected with plasmid expressing HPV-11  E2 protein, Lines 2 and 3 are   U2OS and Hela non-transfected cells. Line 5. Protein size marker.

                 

Figure 2. IF analysis of HPV-11 E2 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-11 E2 protein. All the nuclei were stained by DAPI.  Green color represents nucleus-localized HPV-11 E2 detected by Mab 4H1. IF analysis of E2 protein show similar result also in HeLa cells

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Catalogue #

A2-402-100

Name

Mouse monoclonal antibody to HPV11 E2 (clone 4H1)

Target

HPV-11 E2

Target Description

Recombinant full-length HPV-11 E2-protein produced in E. coli

Alternative Names

Human papillomavirus type 11 E2 protein, HPV11 E2

Clonality

Mouse monoclonal

Clone

4H1

Class

mIgG1

Reactivity

Reacts with native and recombinant HPV-11 E2-protein

Application

ELISA, WB, IF

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

ELISA:

0,067-0,1 µg/ml

WB:

0,9 µg/ml

Purification

Protein G affinity chromatography

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

Shipping in ambient conditions.

Storage

Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.

Background

Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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