Mouse monoclonal antibody to HPV11 E7 (clone 8G6)

Figure 1. Western-Blot analysis of HPV-11 E7 protein. WB was carried out with 8G6 Mab using the lysates of U20S and Hela cells. Lines 1 and 4 are according cells transfected with plasmid expressing HPV-11 E7 protein, Lines 2 and 3 non-transfected U2OS and Hela cells. Line 5. Protein size marker

Figure 2. IP analysis of HPV-11 E7 protein.  Line 1. shows detection of E7 protein by mAB 8G6 in lysate of U20S cells. Line 2. the lower band is precipitated E7, upper bands are light and heavy chain of Mab. Line 3. precipitation without mAB 8G6 Line 4. Non-transfected U2OS cells. Line 5. Protein size marker

Figure 3. IF analysis of HPV-11 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-11 E7 protein. All the nuclei were stained by DAPI.  Green color represents nucleus-localized HPV-11 E7 detected by Mab 8G6. IF analysis of E7 protein show similar result also in HeLa cells

(for reference only; data shown are from a single production batch at release and may not be representative of other batches)