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Figure 1 Western-Blot analysis of HPV-18 E7 protein. WB was carried out with 10A8 Mab using the lysates of U2OS, HeLa and SiHa cells. Line 1: U2OS with plasmid expressing E7 protein, Line 2. Non-transfected U2OS cells. Lines 3 and 4. Lysate of HeLa and SiHa cells respectively. The band in Lane 3 represents positive control (HeLa contains integrated HPV-18). Line 5. Protein size marker.
Figure 2. IP analysis of HPV-18 E7 protein. Line 1. detection of E7 protein by mAb 10A8 in U2OS lysate Line 2. lower band is precipitated E7, upper bands are light and heavy chain of mAb. Line 3. precipitation without mAb 10A8. Line 4. IP exp. with non-transfected U2OS cells. Line 5. IP exp. non-transfected HeLa cells (HeLa contains integrated HPV-18). Line 6. Protein size marker.
Catalogue #
A2-407-100
Name
Mouse monoclonal antibody to HPV18 E7 (clone 10A8)
Target
HPV-18 E7
Target Description
Recombinant full-length HPV-18 E7-protein produced in E. coli
Alternative Names
Human papillomavirus type 18 E7 protein, HPV18 E7
Clonality
Mouse monoclonal
Clone
10A8
Class
mIgG1
Reactivity
Reacts with HPV-18 E7-protein. Mapped to amino acids 1-35
Application
ELISA, WB, IF, IP
Protocol
ELISA:
0,05-0,1 µg/ml
WB:
1 µg/ml
IF:
0,33-1 µg/ml
IP:
2 µg/ml
Purification
Protein G affinity chromatography
Buffer
PBS pH 7.4, with 0.1% sodium azide
Shipping
Shipping in ambient conditions.
Storage
Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.
Background
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins have cellular transforming and trans-activating activities
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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