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Figure 1. Western-Blot analysis of HPV-18 E7 protein. WB was carried out with 8E2 Mab using the lysates of U20S, Hela and SiHa cells. Line 1. U20S with plasmid expressing E7 protein, Line 2. plain U2OS cells. Line 3. Lysate of Hela cells. Hela cells are containing HPV-18 genome sequences expressing HPV E6 and E7 proteins. Line 4. Lysate of SiHa cells. SiHa cells are containing HPV-16 genome sequence, Line 5. Protein size marker
Figure 2. IF analysis of HPV-18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus-localized HPV-18 E7 detected by Mab 8E2. IF analysis of E7 protein show similar result also in HeLa cells
Figure 3. IP analysis of HPV-18 E7 protein. Lane 1: detection of E7 protein, tagged by BPV epitope tag 5E11 by Mab 8E2 in lysate of U2OS cells. Lane 2: the lower band is precipitated E7, upper bands are light and heavy chain of Mab. Lane 3: precipitation without mab 8E2. Lanes 4 and 5: IP exp. with non-transfected U2OS and HeLa cells, respectively. Lower band corresponds to native HPV-18 E7 protein expressed by HPV-18 genome expressed in Hela cells. Lane 6: markerCatalogue #
A2-406-100
Name
Mouse mAb to HPV18 E7 (clone 8E2)
Target
HPV-18 E7
Target Description
Recombinant full-length HPV-18 E7-protein produced in E. coli
Alternative Names
Human papillomavirus type 18 E7 protein, HPV18 E7
Clonality
Mouse monoclonal
Clone
8E2
Class
mIgG1
Reactivity
Reacts with HPV-18 E7-protein. Mapped to amino acids 36-70.
Application
ELISA, WB, IF, IP
Protocol
ELISA:
0,05-0,1 µg/ml
WB:
1 µg/ml
IF:
0,33-10 µg/ml
IP:
2 µg/ml
Purification
Protein G affinity chromatography followed by gel filtration
Concentration
1 mg/ml
Buffer
PBS pH 7.4, with 0.1% sodium azide
QC
LabChip protein analysis, SDS-PAGE, analytical HPLC-SEC, ELISA
Shipping
Shipping in ambient conditions.
Storage
Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.
Background
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins have cellular transforming and trans-activating activities
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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