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Figure 1. Western Blot analysis of anti-BDNF monoclonal antibody 3B2. Lane 1: 10 µl of supernatant was loaded into the gel under reducing conditions. Antibody concentration 5 µg/ml. HRP-conjugated Goat anti-Mouse IgG was used as secondary antibody. Lane 2: Protein size marker
Figure 2. Immunofluorescence detection of hBDNF expression in U2OS cells by anti-BDNF monoclonal antibody 3B2. Antibody concentration 0.33 µg/ml. Goat anti-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for BDNF specific signal and blue for nuclei. proBDNF-expressing U2OS cells (Left); Negative control, non-transfected U2OS cells (Right).(for reference only; data shown are from a single production batch at release and may not be representative of other batches)
Catalogue #
329-100
Name
Mouse monoclonal antibody to hBDNF (clone 3B2)
Target
Human BDNF
Target Description
Recombinant human BDNF protein purified from E. coli
Alternative Names
Abrineurin, neurotrophic factor BDNF precursor form, proBDNF, hBDNF, Brain-Derived Neurotrophic Factor
Uniprot ID
P23560
Clonality
Mouse monoclonal
Clone
3B2
Class
mIgG2b
Reactivity
Human, mouse, rat, guinea pig
Application
ELISA, WB, IF
Protocol
Concentration
1 mg/ml
Buffer
PBS pH 7.4 with 0.1% sodium azide
Shipping
Shipping in ambient conditions.
Storage
Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.
Background
Brain-derived neurotrophic factor (BDNF) plays an important role in activity-dependent synaptic plasticity such as long-term potentiation. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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