Mouse monoclonal antibody to human CDNF (clone 7D6)

Catalogue #

301-100

Name

Mouse monoclonal antibody to human CDNF (clone 7D6)

Target

Human CDNF

Target Description

Recombinant human CDNF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Purified from cell culture supernatant

Alternative Names

ARMETL1, cerebral dopamine neurotrophic factor, ARMET-like protein 1, conserved dopamine neurotrophic factor

Uniprot ID

Q49AH0

Clonality

Mouse monoclonal

Clone

7D6

Class

mIgG1

Reactivity

Human, mouse; others not tested
Binds to the N-terminal part of the human CDNF (aa 25-125)

Application

ELISA, WB, IF

Protocol

ELISA:

(rhCDNF) 0,1-0,2 µg/ml; (rmCDNF) 0,13-0,4 µg/ml

WB:

0,25-1 µg/ml

IF:

0,33-10 µg/ml

Purification

Protein G affinity chromatography

Concentration

1 mg/ml

Buffer

PBS pH 7.4, with 0.1% sodium azide

QC

ELISA, WB

Shipping

Shipped on blue ice.

Storage

Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.

Background

CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta