Mouse monoclonal antibody to hGDNF (clone 3C1)

Catalogue #

315-100

Name

Mouse monoclonal antibody to hGDNF (clone 3C1)

Target

Human GDNF

Target Description

Recombinant human GDNF protein produced using E. coli expression system

Alternative Names

Astrocyte-derived trophic factor, ATF, glial cell line-derived neurotrophic factor, hGDNF

Uniprot ID

P39905

Clonality

Mouse monoclonal

Clone

3C1

Class

mIgG1

Reactivity

Human GDNF

Application

ELISA, WB, IF, IHC

Protocol

ELISA:

50-100 ng/ml

WB:

2-5 µg/ml

IF:

2-10 µg/ml

IHC:

5 µg/ml

Purification

Protein G affinity chromatography followed by gel filtration.

Purity

>90%

Concentration

1 mg/ml

Buffer

PBS pH 7.4, with 0.1% sodium azide

QC

SDS-page, analytical HPLC-SEC, ELISA, WB, LabChip protein analysis

Shipping

Shipping in ambient conditions.

Storage

Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.

Background

GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex.