Mouse monoclonal antibody to human MANF (clone 1G12)

Catalogue #

311-100

Name

Mouse monoclonal antibody to human MANF (clone 1G12)

Target

Human MANF

Target Description

Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant

Alternative Names

ARMET, ARP, mesencephalic astrocyte-derived neurotrophic factor

Uniprot ID

P55145

Clonality

Mouse monoclonal

Clone

1G12

Class

mIgG1

Reactivity

Human MANF

Application

ELISA, WB, IF

Protocol

ELISA:

0,1-0,2 µg/ml

WB:

0,5-2 µg/ml

IF:

1-10 µg/ml

IHC-C:

5 µg/ml

Purification

Protein G affinity chromatography

Concentration

1 mg/ml

Buffer

PBS pH 7.4, with 0.1% sodium azide

QC

ELISA, WB

Shipping

Shipping in ambient conditions.

Storage

Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.

Background

MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta