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Mouse monoclonal antibody to human Ribonuclease 7 (clone 4C4)

Figure 1. Western Blot analysis of RNase 7 antibody 4C4. Human RNase 7 was expressed by CHOEBNALT85 cell line. 10 µl of cell culture supernatant was loaded per lane. Lane 1 non-reduced conditions; Lane 2 Reduced conditions; Lane 3 Prestained Protein Ladder, Naxo 8003

   
 
A.                                 B.
 
Figure 2. Immunofluorescence testing anti-RNase7 monoclonal antibody 4C4. Assay was carried out with U2OS cells expressing RNase 7. Antibody concentration of 0.5 ug/ml was used. Nuclei were counterstained by DAPI. A. U2OS cells expressing RNase 7; B. Negative control (non-transfected U2OS cells)
 
Figure 3. Immunohistochemistry analysis of RNase 7 antibody 4C4. Analysis was performed using paraffin-embedded human skin tissue sample. Anti-RNase 7 antibody 4C4 (dilution 1:500) was used as primary antibody. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization

(for reference only; data shown are from a single production batch at release and may not be representative of other batches)

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Catalogue #

407-100

Name

Mouse monoclonal antibody to human Ribonuclease 7 (clone 4C4)

Target

Human Ribonuclease 7

Target Description

Recombinant human RNase 7 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line

Alternative Names

RNase 7, SAP-2, skin-derived antimicrobial protein 2, RNAS7

Uniprot ID

Q9H1E1

Clonality

Mouse monoclonal

Clone

4C4

Class

mIgG1

Reactivity

Human Ribonuclease7

Application

ELISA, WB, IHC, IF

Protocol

Monoclonal antibody optimal dilution has to be established practically for each antigen and assay format

Concentration

1 mg/ml

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

Shipping in ambient conditions.

Storage

Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.

Background

RNase 7 exhibit potent ribonuclease activity and thus may contribute to the well-known ribonuclease activity of human skin. RNase 7 revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nm) against a vancomycin-resistant Enterococcus faecium (Harder and Schröder, 2002)

References

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This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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