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Figure 1. Western Blot testing of anti-MANF polyclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (1 µg per line) expressed using CHOEBNALT85 cell line.
A.
B.
Figure 2. Immunohistochemical analysis of rabbit polyclonal antibody to human MANF. Analysis was performed using 4-5 µm FFPE human cerebral cortex tissue sections from Alzheimer’s disease patients. Antibody concentration of 10 µg/ml was used. A. MANF staining by polyclonal antibody. B. negative staining.
A. B.
Figure 3. Immunofluorescence detection of human MANF expressed in human U2OS cells by rabbit polyclonal antibody to hMANF. Anti-hMANF antibody dilution in IF experiment was 1 µg/ml. A. MANF-expressing U2OS cells. B. negative control (non-transfected cells).(for reference only; data shown are from a single production batch at release and may not be representative of other batches)
Catalogue #
310-100
Name
Rabbit polyclonal antibody to human MANF
Target
Human MANF
Target Description
Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Alternative Names
ARMET, ARP, mesencephalic astrocyte-derived neurotrophic factor
Uniprot ID
P55145
Clonality
Polyclonal
Class
IgG
Reactivity
Human MANF. No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
Application
ELISA, WB, IF, IHC
Protocol
Buffer
50% saturated ammonium sulfate in PBS
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at +2 °C to +8 °C. As product is (NH4)2SO4precipitate, mix well by pipetting or vortexing prior use
Background
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA-induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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