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Figure 1. Western-Blot detection of human NRTN expressed in CHO cells. Line 1. hNRTN-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 µl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Rb pAb to Chk IgY ab6753, was used as secondary antibody. Antibody recognizes protein dimeric, and monomeric variants.
Catalogue #
307-100
Name
Rabbit pAb to human NRTN
Target
human Neurturin
Target Description
Recombinant human NRTN protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Protein was purified from the cell culture supernatant
Alternative Names
NRTN, NTN, Neurturin
Uniprot ID
Q99748
Clonality
Polyclonal
Class
IgG
Reactivity
Reacts to human NRTN. Antibody doesn’t cross-react to other GDNF-family proteins
Application
ELISA, WB, IF
Protocol
ELISA:
0,01-0,02 µg/ml
WB:
0,2-1 µg/ml
IF:
0,5-2 µg/ml
Purification
Immunogen affinity chromatography
Buffer
50% saturated ammonium sulfate in PBS
Shipping
Shipping in ambient conditions.
Storage
Store at +2 °C to +8 °C. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Background
Neurturin (NTN) is a member of the GDNF family of ligands. GDNF family proteins are distant members of the TGF-beta superfamily
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com