Recombinant anti-human interleukin-6 antibody (clone 12C1)

Catalogue #

R1-646-100

Name

Recombinant anti-human interleukin-6 antibody (clone 12C1)

Target

Recombinant hIL-6 protein

Target Description

Recombinant human IL-6 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.

Alternative Names

Interleukin-6, IL6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, interferon beta-2, IFN-beta-2

Uniprot ID

P05231

Clonality

Human monoclonal

Clone

12C1

Class

hIgG1

Reactivity

Binds to human IL-6. Lack of cross-reactivity to IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-8, IFNγ and TNFα proteins was confirmed with Octet.

Application

ELISA, CLIA

Protocol

Buffer

PBS pH 7.4

Related Products

IL-6 antibody clone 12C1 has been identified as a recommended capture antibody with clone 2G12 (cat# R1-641-100) and clone 2A10 (cat# R1-640-100). The antibody pair 12C1–2G12 was additionally validated using serum samples collected from sepsis patients.

Shipping

Shipping on dry ice.

Storage

Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.

Background

Human interleukin-6 (IL-6) is a cytokine secreted by a broad spectrum of cells such as innate immune cells, B cells, endothelial cells, fibroblasts, astrocytes, epithelial cells, and several malignant cells. It is produced in response to tissue damage or environmental stress, microbial products, viruses or other proinflammatory cytokines. IL-6 is involved in a variety of physiological processes, including the regulation of immune response, inflammation, metabolism, bone metabolism, cancer development and progression. Elevated IL-6 concentrations are associated with several inflammatory diseases and malignancies, e.g., multiple myeloma, rheumatoid arthritis, Crohn’s disease, cardiovascular diseases and osteoporosis. In addition, increased levels of IL-6 have been observed in severe cases of COVID-19. Therefore, monitoring IL-6 levels may provide useful information for disease prognosis and management.