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Figure 1. Simply Blue Safe stained SDS-PAGE analysis of recombinant anti-human interleukin-6 antibody, clone 2G12. 4-12% gel is used for analysis. Lane 1. Recombinant anti-human interleukin-6 antibody, clone 2G12 (-DTT) Lane 2. Size marker Lane 3. Recombinant anti-human interleukin-6 antibody, clone 2G12 (+DTT) Lane 4. Size marker
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet BLI analysis, antibody was loaded on sensor for capture of IL-6 protein.(for reference only; data shown are from a single production batch at release and may not be representative of other batches)
Catalogue #
R1-641-100
Name
Recombinant anti-human interleukin-6 antibody (clone 2G12)
Target
Recombinant hIL-6 protein
Target Description
Recombinant human IL-6 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
Alternative Names
Interleukin-6, IL6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, interferon beta-2, IFN-beta-2
Uniprot ID
P05231
Clonality
Human monoclonal
Clone
2G12
Class
hIgG1
Reactivity
Binds to human IL-6. Lack of cross-reactivity to IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-8, IFNγ and TNFα proteins was confirmed with Octet.
Application
ELISA, CLIA
Protocol
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Related Products
IL-6 antibody clone 2G12 has been identified as a recommended detection antibody with clone 14E2 (cat# R1-648-100) and clone 12C1 (cat# R1-646-100). The antibody pairs 14E2–2G12 and 12C1–2G12 were additionally validated using serum samples collected from sepsis patients.
Shipping
Shipped on dry ice.
Storage
Store in -65...-85 °C. Avoid multiple freeze-thaw cycles.
Background
Human interleukin-6 (IL-6) is a cytokine secreted by a broad spectrum of cells such as innate immune cells, B cells, endothelial cells, fibroblasts, astrocytes, epithelial cells, and several malignant cells. It is produced in response to tissue damage or environmental stress, microbial products, viruses or other proinflammatory cytokines. IL-6 is involved in a variety of physiological processes, including the regulation of immune response, inflammation, metabolism, bone metabolism, cancer development and progression. Elevated IL-6 concentrations are associated with several inflammatory diseases and malignancies, e.g., multiple myeloma, rheumatoid arthritis, Crohn’s disease, cardiovascular diseases and osteoporosis. In addition, increased levels of IL-6 have been observed in severe cases of COVID-19. Therefore, monitoring IL-6 levels may provide useful information for disease prognosis and management.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2026 Icosagen
