Search from website
Search from website
Figure 1. IF testing of anti-MANF monoclonal antibody (341-100). Immunofluorescence detection of human MANF expressed in human U2OS cells by chimeric monoclonal antibody to hMANF (341-100). Anti-hMANF antibody dilution in IF experiment was 1 µg/ml. Upper panel: MANF-expressing U2OS cells. Lower panel: Negative control (non-transfected cells)
A
B
Figure 2: Immunohistochemical analysis of MANF monoclonal antibody (341-100). Analysis was performed using mouse pancreas cryosections. Antibody concentration of 5 µg/ml was used. A. WT mouse pancreas. B. MANF KO mouse pancreas.
Catalogue #
341-100
Name
Recombinant mAb to human MANF (clone 2B8, chicken-mouse IgG2a chimeric antibody)
Target
Human MANF
Target Description
Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Alternative Names
ARMET, ARP, mesencephalic astrocyte-derived neurotrophic factor
Uniprot ID
P55145
Clonality
Mouse monoclonal
Clone
2B8, chicken-mouse IgG2a chimeric antibody
Class
mIgG2a
Reactivity
Human MANF
Application
ELISA, IF
Protocol
ELISA:
0,02-0,1 µg/ml
WB:
Conformational antibody, not suitable for Western Blot application
IF:
1-20 µg/ml
Purification
Protein A affinity chromatography
Buffer
PBS pH 7.4, with 0.1% sodium azide
Shipping
Shipping in ambient conditions.
Storage
Store at -20 °C to -70 °C. Avoid multiple freeze-thaw cycles.
Background
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2026 Icosagen
