Search from website
Search from website
Reagent 007 is a next-generation, peptide-based chemical transfection reagent optimized for efficient delivery of DNA into a wide range of mammalian and insect cell lines, including CHO, HEK293, Sf9, etc.
Designed for use in serum-free and chemically defined media, Reagent 007 offers exceptional performance in protein production and virus-like particle (VLP) generation workflows.
Reagent 007 is ideal for a wide range of applications, including recombinant protein and antibody production, gene expression studies, CRISPR/Cas9 plasmid delivery, pseudotyped virus-like particle (VLP) production, and general biopharmaceutical research and development (R&D).
High transfection efficiency in CHO suspension cells (up to 90%)
Low cytotoxicity and excellent post-transfection cell viability
Broad cell line compatibility: CHO, HEK293, Sf9, and other eukaryotic cells
Stable at -20°C for up to 12 months
Many researchers struggle with inconsistent transfection efficiency, high cytotoxicity, and loss of protein function—especially when working with sensitive cell lines or expressing membrane proteins. Traditional lipid-based transfection reagents often cause cell membrane disruption, lead to reduced cell viability, or interfere with protein localization, compromising downstream applications such as virus-like particle (VLP) assembly, antibody expression, or functional assays. Reagent 007 solves these challenges with its peptide-based, non-lipid formulation, offering gentle but highly efficient delivery of nucleic acids. This minimizes cellular stress, preserves physiological protein trafficking, and enables reliable, high-yield expression in both transient and stable systems. It’s particularly valuable for researchers aiming for high transfection rates in suspension CHO or HEK293 cultures without sacrificing cell health or protein quality.
A unique advantage of Reagent 007 is its ability to preserve native membrane protein trafficking, which is essential for the correct assembly of pseudotyped virus-like particles (VLPs). Many conventional transfection reagents cause protein mislocalization, leading to malformed or non-functional VLPs and decreased productivity. In contrast, Reagent 007 enables accurate surface presentation of envelope proteins, resulting in reliable and high-yield VLP production.
This makes it especially valuable for the development of next-generation vaccines and viral entry models targeting complex membrane-associated proteins such as GPCRs, ion channels, viral spike proteins (e.g., SARS-CoV-2, influenza HA), and tumor-associated antigens. These targets are increasingly central to research in infectious diseases, oncology, neurology, and immunology, as well as in the design of VLP-based delivery systems for RNA therapeutics and gene editing tools. By maintaining membrane protein fidelity, Reagent 007 supports cutting-edge applications where protein conformation, localization, and function are critical to success—from diagnostic assay development to therapeutic VLP manufacturing.
Reagent 007 has demonstrated transfection efficiencies of 70–90% in CHO cells cultured in chemically defined, serum-free suspension media. In Icosagen’s proprietary CHO protein production platform, the reagent maintains high viability over 72 hours post-transfection, as confirmed by Trypan Blue exclusion and FACS analysis.
Transfection Reagent 007 has been successfully adopted by academic and commercial laboratories worldwide for applications in biologic drug development, gene therapy research, and molecular biology. It has been validated in peer-reviewed studies and recognized for its balance of efficiency, cell health, and ease of use.
Arukuusk P et al. New generation of efficient peptide-based vectors, NickFects, for the delivery of nucleic acid. Biochim Biophys Acta. 2013.
Karro K et al. DNA Transfer into Animal Cells Using Stearylated CPP-Based Transfection Reagent. Methods Mol Biol. 2015.
Chemical transfection is a widely used method for delivering nucleic acids into eukaryotic cells using non-viral carriers. Reagent 007 leverages a peptide-based, non-lipid formulation to form stable complexes with DNA or RNA, enabling high-efficiency uptake by cells without the toxicity often associated with cationic lipids or polymers. This method is suitable for both transient and stable expression workflows in research and bioproduction.
© 2025 Icosagen
